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KMID : 0043320080310060758
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Archives of Pharmacal Research
2008 Volume.31 No. 6 p.758 ~ p.763
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Inhibition of Prostaglandin and Nitric Oxide Production in Lipopolysaccharide-treated RAW 264.7 Cells by Tanshinones from the Roots of Salvia miltiorrhiza Bunge
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Jeon Su-Jin
Son Kun-Ho
Kim Yeong-Shik
Choi Yong-Hwan
Kim Hyun-Pyo
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Abstract
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This study examnined the effects of tanshinone derivatives (tanshinone I, cryptotanshinone, 15,16-dihydrotanshinone I) on prostaglandin (PG) and nitric oxide (NO) metabolism in an attempt to establish their anti-inflammatory mechanisms and to present a scientific rationale for the use of Salvia miltiorrhiza (danshen) in inflammatory conditions. From lipopolysaccharidetreated RAW 264.7 cells, cyclooxygenase-2 (COX-2)-mediated production was inhibited by tanshinone I, cryptotanshinone and 15,16-dihydrotanshinone I, while only cryptotanshinone and 15,16-dihydrotanshinone I inhibited inducible NO synthase (iNOS)-mediated NO synthesis at . Particularly, cryptotanshinone was found to be a down-regulator of proinflammatory molecule expression, including COX-2 and iNOS. The electrophoretic mobility shift assay showed that cryptotanshinone and 15,16-dihydrotanshinone I also inhibited the activation of the transcription factors, such as nuclear transcription factor- and activator protein-1. Moreover, cryptotanshinone exhibited in vivo anti-inflammatory activity against carrageenaninduced paw edema in rats. Overall, these results provide additional scientific rationale for the anti-inflammatory use of danshen in Chinese medicine. Especially, cryptotanshinone and 15,16-dihydrotanshinone I are important constituents.
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KEYWORD
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Salvia miltiorrhiza Bunge, Tanshinone, Cryptotanshinone, 15, 16-Dihydrotanshinone, Cyclooxygenase, Inducible nitric oxide synthase, Anti-inflammation
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